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SOX9 (phospho-S181) polyclonal antibody

货号: BS4875

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4875

Product Name :SOX9 (phospho-S181) polyclonal antibody

Swiss-Prot :P48436

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-SOX9 (S181) polyclonal antibody detects endogenous levels of SOX9 protein only when phosphorylated at Ser181.

BiowMW :~ 56 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of Soluble Factors to EMT,

Alternative Name :Transcription factor SOX-9; SOX9;

Immunogen :Synthetic phosphopeptide derived from human SOX9 around the phosphorylation site of Serine 181.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-SOX9 (S181) polyclonal antibody at 1:500 dilution Lane1:Hela lysate treated with PMA(100nM,30min) Lane2:Mouse brain tissue lysate Lane3:Rat liver tissue lysate

Immunohistochemistry (IHC) analyzes of p-SOX9 (S181) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of p-SOX9 (S181) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Sublytic C5b-9 induces glomerular mesangial cell proliferation via ERK1/2-dependent SOX9 phosphorylation and acetylation by enhancing Cyclin D1 in rat Thy-1 nephritis

JournalExperimental And Molecular Medicine

ApplicationIHC,WB

IF8.718

SpeciesRat,Human

PMID33811247
ERK1/2-dependent activity of SOX9 is required for sublytic C5b-9-induced expression of FGF1, PDGFα, and TGF-β1 in rat Thy-1 nephritis

JournalINTERNATIONAL IMMUNOPHARMACOLOGY

ApplicationWB

IF5.6

SpeciesRat

PMID38118314