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Smad3 (phospho-S204) polyclonal antibody

货号: BS4874

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4874

Product Name :Smad3 (phospho-S204) polyclonal antibody

Swiss-Prot :P84022

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC ICC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200 IP: 1:50~1:200

Background :Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Smad3 (S204) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Ser204.

BiowMW :~ 48, 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Cell Cycle Control G1 S Checkpoint,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling ,

Alternative Name :Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Immunogen :Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 204.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Smad3 (S204) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with UV(4h) Lane2:sp2/0 cell lysate treated with UV(4h) Lane3:H9C2 cell lysate treated with UV(4h)

Immunohistochemistry (IHC) analyzes of p-Smad3 (S204) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Astragalus and Paeoniae Radix Rubra extract (APE) inhibits hepatic stellate cell activation by modulating transforming growth factor-β/smad pathway

JournalMolecular Medicine Reports

ApplicationWB,IF

IF2.1

SpeciesRat

PMID25435153