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PR/PGR (phospho-S294) polyclonal antibody

货号: BS4868

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4868

Product Name :PR/PGR (phospho-S294) polyclonal antibody

Swiss-Prot :P06401

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The effects of progesterone are mediated by two functionally different isoforms of the progesterone receptor, PR-A and PR-B, which are transcribed from distinct, estrogen inducible promoters within a single copy of the PR gene. The PR-A and PR-B proteins are 90 kDa and 118 kDa respectively; the first 164 amino acids of PR-B are absent in PR-A. Progesterone bound PR-A and PR-B have different transcription activation properties. Specifically, PR-B functions as a transcriptional activator in most cell and promoter contexts, while PR-A is transcriptionally inactive and functions as a strong ligand dependent transdominant repressor of steroid hormone receptor transcriptiona activity. An inhibitory domain (ID), which maps to the amino terminus of the receptor, exists within both PR isoforms.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-PR/PGR (S190) polyclonal antibody detects endogenous levels of Progesterone Receptor protein only when phosphorylated at Ser190.

BiowMW :~ 90 kDa (PR-A) ~ 118 kDa (PR-B)

Note :For research use only, not for use in diagnostic procedure.

Pathway :Nuclear Receptor Signaling,

Alternative Name :NR3C3; PGR; PRGR; Progesterone receptor

Immunogen :Synthetic phosphopeptide derived from human PGR around the phosphorylation site of Serine 294.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-PGR (S294) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF(0.1ng/ML,30mins) Lane2:sp2/0 cell lysate treated with EGF(0.1ng/ML,15mins) Lane3:PC12 cell lysate treated with PMA(100nM,15mins)