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PP2A-α polyclonal antibody（KU）

货号: BS4867N

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS4867N

Product Name :PP2A-α polyclonal antibody（KU）

Swiss-Prot :P67775

Host :Rabbit

Reactivity :Rat,Mouse,Human

Applications :WB IP IF

Application_all :WB: 1:1000~1:2000 IP: 1:50~1:200 IF: 1:100~1:500

Background :PP2A is the major phosphatase for microtubule-associated proteins (MAPs).PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase.Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity).Can dephosphorylate SV40 large T antigen and p53/TP53.Activates RAF1 by dephosphorylating it at 'Ser-259'.Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint.Mediates dephosphorylation of MYC; promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC.Mediates dephosphorylation of FOXO3; promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PP2A-α polyclonal antibody detects endogenous levels of PP2A-α protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of Soluble Factors to EMT,

Alternative Name :Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C

Immunogen :Synthetic peptide, corresponding to Human PP2A-α.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PP2A-α polyclonal antibody at 1:1000 dilution Lane1:The liver tissue lysate of mouse(30ug) Lane2:The heart tissue lysate of Rat(30ug) Lane3:K562 whole cell lysate(30ug)

Immunofluorescence analysis of K562 cells using PP2A-α pAb at dilution of 1:200 (40x lens).