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p47-phox (phospho-S370) polyclonal antibody

货号: BS4852

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4852

Product Name :p47-phox (phospho-S370) polyclonal antibody

Swiss-Prot :P14598

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC ICC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200 IP: 1:50~1:200

Background :The heredity disease chronic granulomatous disease (CGF) has been linked to mutations in p47-phox and p67-phox. The cytosolic proteins p47-phox and p67-phox, also designated neutrophil cytosol factor (NCF)1 and NCF2, respectively, are required for activation of the superoxide-producing NADPH oxidase in neutrophils and other phagocytic cells. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with cytochrome b558 and the small G protein Rac to form the functional enzyme complex. Both p47-phox and p67-phox contain two Src homology 3 (SH3) domains. The C-terminal SH3 doamin of p67-phox has been shown to interact with the proline rich domain of p47-phox, suggesting that p47-phox may faciliate the transport of p67-phox to the membrane.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-p47-phox (S370) polyclonal antibody detects endogenous levels of p47-phox protein only when phosphorylated at Ser370.

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1; NOXO2; SH3PXD1A

Immunogen :Synthetic phosphopeptide derived from human p47-phox around the phosphorylation site of Serine 370.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-p47-phox (S370) polyclonal antibody at 1:500 dilution Lane1:Hela cell treated with serum starvation(24h) Lane2:Raw264.7 cell treated with serum starvation(24h) Lane3:H9C2 cell treated with serum starvation(24h)

Immunohistochemistry (IHC) analyzes of p-p47-phox (S370) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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