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MEK1 (phospho-T286) polyclonal antibody

货号: BS4844

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4844

Product Name :MEK1 (phospho-T286) polyclonal antibody

Swiss-Prot :Q02750

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB:1:500~1:1000 IHC:1:50~1:200

Background :MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-MEK1 (T286) polyclonal antibody detects endogenous levels of MEK1 protein only when phosphorylated at Thr286.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Warburg Effect,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1

Immunogen :Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Threonine 286.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-MEK1 (T286) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with PMA(100nM,30mins) Lane2:NIH-3T3 cell lysate treated with UV(24h) Lane3:PC12 cell lysate treated with PMA(100nM,15mins)

Immunohistochemistry (IHC) analyzes of p-MEK1 (T286) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of p-MEK1 (T286) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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