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MARCKS (phospho-S163) polyclonal antibody

货号: BS4799

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4799

Product Name :MARCKS (phospho-S163) polyclonal antibody

Swiss-Prot :P29966

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa; however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC α and PKC ε, but not PKC δ, are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-MARCKS (S163) polyclonal antibody detects endogenous levels of MARCKS only when phosphorylated at Ser163.

BiowMW :~ 32, 75, 87 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Protein Kinase C Signaling,

Alternative Name :Myristoylated alanine-rich C-kinase substrate; MARCKS; Protein kinase C substrate, 80 kDa protein, light chain; 80K-L protein; PKCSL; MARCKS; MACS; PRKCSL

Immunogen :Synthetic phosphopeptide derived from human MARCKS around the phosphorylation site of Serine 163.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-MARCKS (S163) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with PMA Lane2:sp2/0 cell lysate treated with PMA Lane3:PC12 cell lysate treated with PMA