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EGFR (Phospho-Tyr1110) polyclonal antibody

货号: BS4796

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4796

Product Name :EGFR (Phospho-Tyr1110) polyclonal antibody

Swiss-Prot :P00533

Host :Rabbit

Reactivity :Human,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation. Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling. Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation. The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :EGFR (Phospho-Tyr1110) polyclonal antibody detects endogenous levels of EGFR protein only when phosphorylated at Tyr1110.

BiowMW :~ 175 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,Jak Stat ：IL-6 Receptor Signaling,

Alternative Name :Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; EGFR; ERBB, ERBB1, HER1

Immunogen :Synthetic phosphopeptide derived from human EGFR around the phosphorylation site of Tyrosine 1110.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of EGFR (Phospho-Tyr1110) polyclonal antibody at 1:500 dilution LaneA:PMVEC whole cell lysate(40ug) LaneB:HCC827 whole cell lysate(40ug)