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Dok-2 (phospho-Y299) polyclonal antibody（KU）

货号: BS4786

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4786

Product Name :Dok-2 (phospho-Y299) polyclonal antibody（KU）

Swiss-Prot :O60496

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that p62 Dok-1 is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, is a member of a class of “docking” proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF. Dok-2 (also designated p56 Dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Dok-2 (Y299) polyclonal antibody detects endogenous levels of Dok-2 protein when phosphorylated at Tyr299.

BiowMW :~ 56 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Docking protein 2; Downstream of tyrosine kinase 2; p56(dok-2); DOK2

Immunogen :Synthetic phosphopeptide derived from human Dok-2 around the phosphorylation site of Tyrosine 299.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Dok-2 (Y299) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug) Lane4:3T3-L1 whole cell lysate(40ug)