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CPI-17 (phospho-T38) polyclonal antibody（KU）

货号: BS4780

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4780

Product Name :CPI-17 (phospho-T38) polyclonal antibody（KU）

Swiss-Prot :Q96A00

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-CPI-17 (T38) polyclonal antibody detects endogenous levels of CPI-17 polyclonal antibody protein only when phosphorylated at Thr38.

BiowMW :~ 22 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17; CPI17; PPP1INL; PPP1R14A

Immunogen :Synthetic phosphopeptide derived from human CPI-17 around the phosphorylation site of Threonine 38.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-CPI-17 (T38) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:The Brain tissue lysate of Mouse(20ug)

Immunohistochemistry (IHC) analyzes of p-CPI-17 (T38) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Altered Contractile Phenotypes of Intestinal Smooth Muscle in Mice Deficient in Myosin Phosphatase Target Subunit 1

JournalGastroenterology

ApplicationWB

IF17.373

SpeciesMouse

PMID