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Cortactin (phospho-Y466) polyclonal antibody（KU）

货号: BS4779

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4779

Product Name :Cortactin (phospho-Y466) polyclonal antibody（KU）

Swiss-Prot :Q14247

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Cortactin (Y466) polyclonal antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr466.

BiowMW :~ 85 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Contribution of ECM and Cytoskeletal Factors to EMT,Adherens Junction signaling,

Alternative Name :Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; EMS1

Immunogen :Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of p-Cortactin (Y466) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate Lane4:Hela whole cell lysate