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c-Met (phospho-Y1234) polyclonal antibody（KU）

货号: BS4752

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS4752

Product Name :c-Met (phospho-Y1234) polyclonal antibody（KU）

Swiss-Prot :P08581

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :c-Met, a member of the tyrosine kinase superfamily, is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF). The mature c-Met protein is a disulfide-linked heterodimer with Mr=190 kDa composed of a heavily glycosylated alpha subunit that is completely extracellular in localization, and a beta subunit comprised of an extracellular ligand binding domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Cells expressing c-Met include epithelial cells, endothelial cells, blood cells of various types, and glomerular mesenchymal cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-c-Met (Y1234) polyclonal antibody detects endogenous levels of c-Met protein only when phosphorylated at Tyr1234.

BiowMW :~ 156 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met; MET

Immunogen :Synthetic phosphopeptide derived from human c-Met around the phosphorylation site of Tyrosine 1234.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Met (Y1234) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:MCF-7 treated with insulin(100nM/mL,5 minutes) whole cell lysate(40ug) Lane3:MCF-7 treated with insulin(100nM/mL,10 minutes) whole cell lysate(40ug)

PKG II reverses HGF-triggered cellular activities by phosphorylating serine 985 of c-Met in gastric cancer cells

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID27147579
Effects of PHA-665752 and vemurafenib combination treatment on in vitro and murine xenograft growth of human colorectal cancer cells with BRAFV600E mutations

JournalOncology Letters

ApplicationIHC

IF1.871

SpeciesMouse

PMID29456739