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RPS6 (phospho-S235) polyclonal antibody（KU）

货号: BS4745

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4745

Product Name :RPS6 (phospho-S235) polyclonal antibody（KU）

Swiss-Prot :P62753

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB: 1:500~1:1000 IF: 1:50~1:200

Background :Ribosomal protein S6 is a component of the small 40S ribosomeal subunit and belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Ribosomal Protein S6 (S235) polyclonal antibody detects endogenous levels of Ribosomal Protein S6 protein only when phosphorylated at Ser235.

BiowMW :~ 29 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Immune Cell Signaling,T cell receptor signaling,

Alternative Name :40S ribosomal protein S6; Phosphoprotein NP33; RPS6; OK/SW-cl.2

Immunogen :Synthetic phosphopeptide derived from human Ribosomal Protein S6 around the phosphorylation site of Serine 235.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-RPS6 (S235) pAb at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:Panc1 whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:EC9706 whole cell lysate(40ug)