产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

MEK1/2 (phospho-S218/222) polyclonal antibody

货号: BS4733

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4733

Product Name :MEK1/2 (phospho-S218/222) polyclonal antibody

Swiss-Prot :Q02750/P36507

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-MEK1/2 (S218/222) polyclonal antibody detects endogenous levels of MEK-1 protein only when phosphorylated at Ser218. This antibody also detects endogenous levels of MEK-2 protein only when phosphorylated at Thr222.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Warburg Effect,Warburg Effect,G Protein-coupled Receptors Signaling to MAPK Erk,MAPK Erk in Growth and Differentiation Pathway,MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 2; MAP kinase kinase 2; MAPKK 2; ERK activator kinase 2; MAPK/ERK kinase 2; MEK 2; MAP2K2; MEK-2; MKK2; PRKMK2

Immunogen :Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Serine 218.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-MEK1/2 (S218/222) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate treated with PMA(100nM,30mins) Lane2:Raw264.7 cell lysate treated with PMA(100nM,30mins) Lane3:NIH-3T3 cell lysate treated with PMA(100nM,30mins) Lane4: PC12 cell lysate treated with PMA(100nM,30mins)

Immunohistochemistry (IHC) analyzes of p-MEK1/2 (S218/222) pAb in paraffin-embedded human breast carcinoma tissue.

Antiinflammation Effects and Mechanisms Study of Geniposide on Rats with Collagen‐Induced Arthritis

JournalPhytotherapy Research

ApplicationWB

IF3.766

SpeciesRat

PMID28127805
IFN-γ enhances the therapeutic efficacy of MSCs-derived exosome via miR-126-3p in diabetic wound healing by targeting SPRED1

JournalJournal of Diabetes

ApplicationWB

IF4.5

SpeciesMouse

PMID37646268
EGFR-MEK1/2 cascade negatively regulates bactericidal function of bone marrow macrophages in mice with Staphylococcus aureus osteomyelitis

JournalPLoS Pathogens

ApplicationIF

IF5.5

SpeciesMouse

PMID39102432