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Cofilin (phospho-S3) polyclonal antibody（KU）

货号: BS4716

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4716

Product Name :Cofilin (phospho-S3) polyclonal antibody（KU）

Swiss-Prot :P23528

Host :Rabbit

Reactivity :Human

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Cofilin (S3) polyclonal antibody detects endogenous levels of Cofilin protein only when phosphorylated at Ser3.

BiowMW :~ 19 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phosphoinosotode Signaling,Regulation of Actin Dynamics,Tumor Angiogenesis,Regulation of Microtubule Dynamics,B cell receptor signaling,

Alternative Name :Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform; CFL1; CFL

Immunogen :Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Cofilin (S3) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Cofilin (S3) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

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