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mTOR (phospho-S2448) polyclonal antibody

货号: BS4706

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4706

Product Name :mTOR (phospho-S2448) polyclonal antibody

Swiss-Prot :P42345

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :mTOR, or FKBP12-rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 245-kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-mTOR (S2448) polyclonal antibody detects endogenous levels of mTOR protein when phosphorylated at Ser2448.

BiowMW :~ 289 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phosphoinosotode Signaling,PI3K AKT signaling,Hypoxia Signaling,Warburg Effect,Insulin &Glucose Signaling,Glutamine Metabolism,MTOR signaling,ErbB HER Signaling,

Alternative Name :Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Immunogen :Synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Serine 2442.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-mTOR (S2448) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with insulin(100nM,30mins) Lane2:Raw264.7 cell lysate treated with insulin(100nM,30mins) Lane3:PC12 cell lysate treated with insulin(100nM,30mins)

Immunohistochemistry (IHC) analyzes of p-mTOR (S2448) pAb in paraffin-embedded human brain tissue.

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