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Rb Recombinant Rabbit mAb (KU)

货号: BS46841

价格：￥ 1200

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS46841

Product Name :Rb Recombinant Rabbit mAb (KU)

Swiss-Prot :P13405

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, ICC/IF, FC, IP

Application_all :WB: 1:1000<br />IHC: 1:20-1:500<br />ICC/IF: 1:100<br />FC: 1:20<br />IP: 1:20

Background :Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).

Product :Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Purification&Purity :Affinity Purification

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG

BiowMW :106 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Rb; pRb; Rb-1

Immunogen :Recombinant protein of mouse Rb

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of Rb in C6,Jurkat,Hela cell lysates using Rb antibody(1:1000 diluted).

免疫沉淀（IP）

免疫组织化学（IHC）