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NPM (phospho-T234) polyclonal antibody

货号: BS4656

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4656

Product Name :NPM (phospho-T234) polyclonal antibody

Swiss-Prot :P06748

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-NPM (T234) polyclonal antibody detects endogenous levels of NPM protein only when phosphorylated at Thr234.

BiowMW :~ 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin; NPM1; NPM

Immunogen :Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 234.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-NPM1 (T234) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with colchicine(0.2µg/ML,24h) Lane2:Raw264.7 cell treated with colchicine(0.2µg/ML,24h) Lane3:PC12 cell lysate treated with colchicine(0.2µg/ML,24h)

Immunohistochemistry (IHC) analyzes of p-NPM (T234) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.