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ATF2 (phospho-S498) polyclonal antibody

货号: BS4654

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4654

Product Name :ATF2 (phospho-S498) polyclonal antibody

Swiss-Prot :P15336

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-ATF2 (S498) polyclonal antibody detects endogenous levels of ATF2 protein only when phosphorylated at Ser498.

BiowMW :~ 55 to 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :Cyclic AMP-dependent transcription factor ATF-2; cAMP-dependent transcription factor ATF-2; Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1; ATF-2; CREB2; CREBP1

Immunogen :Synthetic phosphopeptide derived from human ATF2 around the phosphorylation site of Serine 498.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-ATF2 (S480) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with UV(4h) Lane2:sp2/0 cell lysate treated with UV(4h) Lane3:MCF-7 cell lysate treated with UV(4h)

Immunohistochemistry (IHC) analyzes of p-ATF2 (S498) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of p-ATF2 (S498) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.