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NOS3 (phospho-T494) polyclonal antibody(KU)

货号: BS4636
价格 1350
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS4636
Product Name :NOS3 (phospho-T494) polyclonal antibody(KU)
Swiss-Prot :P29474
Host :Rabbit
Reactivity :Human
Applications :WB IHC
Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200
Background :NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-NOS3 (T494) polyclonal antibody detects endogenous levels of NOS3 protein when phosphorylated at Thr494. (removal of the initiator methionine)
BiowMW :~ 140 kDa
Note :For research use only, not for use in diagnostic procedure.
Pathway :Hypoxia Signaling,Tumor Angiogenesis,
Alternative Name :Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3
Immunogen :Synthetic phosphopeptide derived from human NOS3 around the phosphorylation site of Threonine 494.
Conjugate :Unconjugated
Modification :Phosphorylation
Western blot (WB) analysis of p-NOS3 (T494) pAb at 1:1000 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:SGC7901 treated with H2O2(100nM/ml,5 minutes) whole cell lysate(40ug) Lane3:SGC7901 treated with H2O2(100nM/ml,10 minutes) whole cell lysate(40ug)

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com