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MAP3K4 Recombinant Rabbit mAb (KU)

货号: BS46184

价格：￥ 1200

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS46184

Product Name :MAP3K4 Recombinant Rabbit mAb (KU)

Swiss-Prot :Q9Y6R4

Host :Rabbit

Reactivity :Human

Applications :WB,IHC,ICC/IF,FC

Application_all :WB: 1:1000<br />IHC: 1:20<br />ICC/IF: 1:50<br />FC: 1:20

Background :The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N-terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Several alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, May 2014]

Product :Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Purification&Purity :Affinity Purification

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG

BiowMW :200 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MTK1; MEKK4; MEKK 4; MAPKKK4; PRO0412

Immunogen :A synthetic peptide of human MAP3K4

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of MAP3K4 in HL-60,U251 cell lysates using MAP3K4 antibody(1:1000 diluted).

免疫沉淀（IP）

免疫组织化学（IHC）