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IRF3 (Phospho-S385) polyclonal antibody

货号: BS4609

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4609

Product Name :IRF3 (Phospho-S385) polyclonal antibody

Swiss-Prot :Q14653

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IHC IF/ICC

Application_all :WB: 1:500~1:1000 IHC: 1:100~1:200 IF/ICC: 1:100~1:500

Background :Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3γ p48 and IFN consensus sequence-binding protein (ICSBP).

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IRF3 (Phospho-S385) polyclonal antibody detects endogenous levels of IRF3 protein only when phosphorylated at Ser385.

BiowMW :~ 47 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Interferon regulatory factor 3; IRF-3

Immunogen :KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S385 of human IRF3 protein.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot analysis of IRF3 (Phospho-S385) expression in HEK293T (A), HT29 (B) whole cell lysates.

Immunohistochemical analysis of IRF3 (Phospho-S385) staining in human breast cancer formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of IRF3 (Phospho-S385) staining in HeLa cells.

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