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NIPA (phospho-S354) polyclonal antibody（KU）

货号: BS4601

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4601

Product Name :NIPA (phospho-S354) polyclonal antibody（KU）

Swiss-Prot :Q86WB0

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Entry into mitosis is essentially driven by cyclin B1 which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear Interaction Partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-NIPA (S354) pAb detects endogenous levels of NIPA protein only when phosphorylated at Ser354.

BiowMW :~ 60-65 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1; ZC3HC1; NIPA; HSPC216

Immunogen :Synthetic phosphopeptide derived from human NIPA around the phosphorylation site of Serine 354

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-NIPA (S354) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40µg) Lane2:SK-OVCAR3 whole cell lysate(40µg) Lane3:MCF-7 whole cell lysate(40µg) Lane4:C6 whole cell lysate(40µg) Lane5:CT-26 whole cell lysate(40µg)