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Smad2/3 (phospho-T8) polyclonal antibody（KU）

货号: BS4590

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4590

Product Name :Smad2/3 (phospho-T8) polyclonal antibody（KU）

Swiss-Prot :P84022/Q15796

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Smad2/3 (T8) polyclonal antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at Thr8.

BiowMW :~ 55, 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Cell Cycle Control G1 S Checkpoint,Contribution of Soluble Factors to EMT,ESC Pluripotency and Differentiation Signaling Pathway,hippo Signaling,hippo Signaling,

Alternative Name :Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2

Immunogen :Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Smad2/3 (T8) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40µg) Lane2:Hela whole cell lysate(40µg) Lane3:The Lung tissue lysate of Mouse(40µg) Lane4:SGC7901 whole cell lysate(40µg) Lane5:H9C2 whole cell lysate(40µg)