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PKC ε (phospho-S729) polyclonal antibody（KU）

货号: BS4583

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4583

Product Name :PKC ε (phospho-S729) polyclonal antibody（KU）

Swiss-Prot :Q02156

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, ∂, é, Ω, h, †, λ/î, µ and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC ∂ and é are independent of Ca++. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.

Specificity :PKC ε (phospho-S729) polyclonal antibody detects endogenous levels of PKC ε protein only when phosphorylated at Ser729.

BiowMW :~ 76~85 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Protein kinase C epsilon type; nPKC-epsilon; PRKCE; PKCE

Immunogen :Synthetic phosphopeptide derived from human PKC ε around the phosphorylation site of Serine 729

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of PKC ε (phospho-S729) polyclonal antibody at 1:500 dilution Lane1:HEK293 whole cell lysate(40µg) Lane2:U-87MG whole cell lysate(40µg) Lane3:SGC7901 whole cell lysate(40µg) Lane4:SGC7901 treated with PMA(50ng/ml)for 5 minutes then repair for 10 minutes whole cell lysate(40µg) Lane5:SGC7901 treated with PMA(50ng/ml)for 5 minutes then repair for 30 minutes whole cell lysate(40µg)

Ginseng oligosaccharides (GSO) inhibit C48/80-stimulated pseudoallergic mechanisms through the PLC/Ca2+/PKC/MAPK/c-Fos signaling pathway

JournalJournal of Functional Foods

ApplicationWB

IF5.223

SpeciesRat

PMID