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BRG1 Recombinant Rabbit mAb (KU)

货号: BS45549

价格：￥ 1200

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS45549

Product Name :BRG1 Recombinant Rabbit mAb (KU)

Swiss-Prot :Q3TKT4

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB,IHC,ICC/IF,FC,IP,ChIP

Application_all :WB: 1:2000-1:10000 IHC: 1:20-1:100 ICC/IF: 1:50 FC: 1:20 IP: 1:20-1:50 ChIP: 1:20

Background :Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).

Product :Store at -20°C. Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.

Purification&Purity :Affinity Purification

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG

BiowMW :220 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Brg1; SW1/SNF; HP1-BP72; SNF2beta; b2b692Clo; b2b508.1Clo

Immunogen :A synthetic peptide of mouse BRG1

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of BRG1 in 293T,Hela,HepG2，A594 cell lysates using BRG1 antibody(1:1000 diluted).

免疫沉淀（IP）

免疫组织化学（IHC）