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RPS6 (phospho-S240) polyclonal antibody（KU）

货号: BS4359

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4359

Product Name :RPS6 (phospho-S240) polyclonal antibody（KU）

Swiss-Prot :P62753

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Ribosomal protein S6 is a component of the small 40S ribosomeal subunit and belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Ribosomal Protein S6 (S240) polyclonal antibody detects endogenous levels of Ribosomal Protein S6 protein only when phosphorylated at Ser240

BiowMW :~ 29 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Immune Cell Signaling,T cell receptor signaling,

Alternative Name :40S ribosomal protein S6; Phosphoprotein NP33; RPS6; OK/SW-cl.2

Immunogen :Synthetic phosphopeptide derived from human Ribosomal Protein S6 around the phosphorylation site of Serine 240.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of RPS6 (phospho-S240) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate(40μg) Lane2:Hela treated with serum starved( 24h) whole cell lysate(40μg) Lane3:PC12 whole cell lysate(40μg) Lane4:PC12 treated with serum starved( 24h) whole cell lysate(40μg)

Immunohistochemistry (IHC) analyzes of p-RPS6 (S240) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Western blot (WB) analysis of RPS6 (phospho-S240) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with insulin(20ng/ml) for 15 minutes whole cell lysate(40ug) Lane3:HEK293T treated with insulin(20ng/ml) for 30 minutes whole cell lysate(40ug)

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