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RyR2 (phospho-S2808) polyclonal antibody

货号: BS4358

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4358

Product Name :RyR2 (phospho-S2808) polyclonal antibody

Swiss-Prot :Q92736

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Dihydropyridine receptor (DHPR) is a surface membrane protein critical for the excitation-contraction coupling of striated muscle. DHPR and the sarcoplasmic reticulum ryanodine receptor (RyR) are two key components of the intracellular junctions, where depolarization of the surface membrane is converted into the release of Ca2+ from internal stores. The α1-subunit of the DHPR contains a cytoplasmic loop which is thought to be involved in the interactions with RyR. Phosphorylation of the DHPR α1-subunit is also thought to play a role in the functional interaction of DHPR and RyR. Mutation in DHPR α1 results in excitation-contraction uncoupling, leading to muscular dysgenesis, a complete inactivity in developing skeletal muscles. Cells that do not express RyR also lack excitation-contraction coupling and exhibit a severalfold reduction in Ca2+ current density.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-RyR2 (S2808)pAb detects endogenous levels of RyR2 protein only when phosphorylated at Ser2808

BiowMW :~ 565 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Ryanodine receptor 2; RYR-2; RyR2; hRYR-2; Cardiac muscle ryanodine receptor; Cardiac muscle ryanodine receptor-calcium release channel; Type 2 ryanodine receptor; RYR2

Immunogen :Synthetic phosphopeptide derived from human RyR2 around the phosphorylation site of Serine 2808.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-RyR2 (S2808) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:U-87MG whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-RyR2 (S2808) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Knockout of M-LP/Mpv17L, a newly identified atypical PDE, induces physiological afferent cardiac hypertrophy in mice

JournalTRANSGENIC RESEARCH

ApplicationWB

IF3

SpeciesMouse

PMID37851308