Product Name :PKC-pan (αT497) polyclonal antibody
Swiss-Prot :P17252/P05771/Q05655/Q02156/P05129/P24723/Q04759/Q05513
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:500~1:1000
IHC: 1:50~1:200
Background :Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, βII and γ) and novel (n) PKC isoforms (δ, ε, ζ, η and θ). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca2+.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-PKC-pan (αT497) polyclonal antibody detects endogenous levels of PKC PKC α, β I, β II, γ, δ, ε, η, ζ and θ isoforms only when phosphorylated at a residue homologous to threonine 497 of human PKCα.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Phosphoinosotode Signaling,Phospholipase Signaling,Protein Kinase C Signaling,Inhibition of Apoptosis,Insulin &Glucose Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,Mitochondrial Control of Apoptosis,
Alternative Name :Protein kinase C alpha type; PKC-A; PKC-alpha; PRKCA; PKCA; PRKACA; Protein kinase C beta type; PKC-B; PKC-beta; PKCB; PRKCB1; PRKCB; Protein kinase C gamma type; PKC-gamma; PKCG; PRKCG; Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; Sphingosine-dependent protein kinase-1; SDK1; PRKCD; Protein kinase C epsilon type; nPKC-epsilon; PRKCE; PKCE; Protein kinase C zeta type; nPKC-zeta; PRKCZ; PKC2; PKC zeta; Protein kinase C theta type; nPKC-theta; PRKCQ; PRKCT
Immunogen :Synthetic phosphopeptide derived from human PKC-pan around the phosphorylation site of Threonine 497.
Modification :Unmodification
