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MYPT1 (phospho-T853) polyclonal antibody（KU）

货号: BS4332

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4332

Product Name :MYPT1 (phospho-T853) polyclonal antibody（KU）

Swiss-Prot :O14974

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. MYPT1 promotor contains one Sp1 transcription factor binding site, suggesting that MYPT1 is a housekeeping gene. Myotonic dystrophy protein kinase phosphorylates MYPT1 at Tyrosine 654 to regulate Myosin II phosphorylation. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphoryl-ates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated Myosin light chain to MYPT1. PP1c interacts with at least four binding sties on the amino-terminus of MYPT1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MYPT1 (phospho-T853) polyclonal antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.

BiowMW :~ 140 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein phosphatase 1 regulatory subunit 12A; Myosin phosphatase-targeting subunit 1; Myosin phosphatase target subunit 1; Protein phosphatase myosin-binding subunit; PPP1R12A

Immunogen :Synthetic peptide derived from human MYPT1 around the phosphorylation site of T853.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of p-MYPT1 (T853) pAb at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug)

The influence of fasudil on the epithelial-mesenchymal transdifferentiation of renal tubular epithelial cells from diabetic rats

JournalBiomedicine & Pharmacotherapy

ApplicationWB

IF6.529

SpeciesRat

PMID
Benidipine Protects Kidney through Inhibiting ROCK1 Activity and Reducing the Epithelium-Mesenchymal Transdifferentiation in Type 1 Diabetic Rats

JournalJournal of Diabetes Research

ApplicationWB,IHC

IF4.011

SpeciesRat

PMID24364038
MYPT1 inhibits the metastasis of renal clear cell carcinoma via the MAPK8/N-cadherin pathway

JournalFEBS Open Bio

ApplicationWB

IF2.792

SpeciesHuman

PMID36106411