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LAT (phospho-Y191) polyclonal antibody（KU）

货号: BS4324

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4324

Product Name :LAT (phospho-Y191) polyclonal antibody（KU）

Swiss-Prot :O43561

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and 191 enables the binding of Grb2, Gads/SLP-76, PLCgamma1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-LAT (Y191) polyclonal antibody detects endogenous levels of human LAT (Isoform Short) only when phosphorylated at Tyr191, and detects LAT (Isoform Long) only when phosphorylated at Tyr220.

BiowMW :~ 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38; LAT

Immunogen :Synthetic phosphopeptide derived from human LAT (Isoform Short) around the phosphorylation site of Tyrosine 191.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of p-LAT (Y191) pAb at 1:1000 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:RAW264.7 whole cell lysate(40ug) Lane3:K562 whole cell lysate(20ug) Lane4:Myla2059 whole cell lysate(20ug) Lane5:HuT78 whole cell lysate(20ug)