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JNK1/2/3 (phospho-T183/Y185) polyclonal antibody（KU）

货号: BS4322

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4322

Product Name :JNK1/2/3 (phospho-T183/Y185) polyclonal antibody（KU）

Swiss-Prot :P45983/P45984/P53779

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-JNK1/2/3 (T183/Y185) polyclonal antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at Thr183 and Tyr185.

BiowMW :~ 46,54 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :JNK 46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 8; MAPK 9; MAPK10; MAPK8; MAPK9; MGC50974; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9

Immunogen :Synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Threonine 183 and Tyrosine 185.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-JNK1/2/3 (T183/Y185) polyclonal antibody at 1:500 dilution Lane1:MCF-7 cell lysate treated with UV Lane2:sp2/0 cell lysate treated with UV Lane3:PC12 cell lysate treated with UV

Western blot (WB) analysis of JNK1/2/3 (phospho-T183/Y185) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV (15min) whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:NIH-3T3 treated with UV (15min) whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug) Lane6:H9C2 treated with UV (4h) whole cell lysate(40ug)

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