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Phospho-IRE1 (Ser724) Recombinant Rabbit mAb (KU)

货号: BS43150

价格：￥ 1200

货期：现货（1-2个工作日）

25ul 50ul 100ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS43150

Product Name :Phospho-IRE1 (Ser724) Recombinant Rabbit mAb (KU)

Swiss-Prot :O75460

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55-60kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HCK;JTK9;p59Hck;p61Hck

Immunogen :Recombinant fusion protein of human HCK(NP_001165600.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using HCK antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.