产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PRAS40 (phospho-T246) polyclonal antibody（KU）

货号: BS4288

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4288

Product Name :PRAS40 (phospho-T246) polyclonal antibody（KU）

Swiss-Prot :Q96B36

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Akt, also known as protein kinase B, is one of the major downstream targets of the phosphatidylinositol 3-kinase pathway. This protein kinase has been implicated in insulin signaling, stimulation of cellular growth, inhibition of apoptosis and transformation of cells. The proline-rich Akt substrate PRAS40, also designated AKT1S1, becomes phosphorylated by activated Akt on Ser or Thr residues in the motif RXRXX(S/T). Phosphorylated PRAS40 subsequently binds 14-3-3 in a sequence-specific manner, thereby inducing such changes as alteration of protein subcellular localization and regulation of intrinsic enzymatic activity. Studies also suggest that PRAS40 phosphorylation and its interaction with pAkt and 14-3-3 may play an important role in neuroprotection mediated by NGF in apoptotic neuronal cell death after cerebral ischemia. PRAS40 maps to human chromosome 19q13.33.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-PRAS40 (T246) polyclonal antibody detects endogenous levels of PRAS40 only when phosphorylated at Thr246.

BiowMW :~ 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :PI3K AKT signaling,Translational Contral elF4 and p70 S6 Kinase,Insulin &Glucose Signaling,MTOR signaling,

Alternative Name :Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate; AKT1S1; PRAS40

Immunogen :Synthetic phosphopeptide derived from human PRAS40 around the phosphorylation site of Threonine 246.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-PRAS40 (T246) polyclonal antibody at 1:500 dilution Lane1:LO2 cell lysate Lane2:Raw264.7 cell lysate Lane3:NIH-3T3 cell lysate