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IRS-1 (phospho-S323) polyclonal antibody（KU）

货号: BS4271

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4271

Product Name :IRS-1 (phospho-S323) polyclonal antibody（KU）

Swiss-Prot :P35568

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-IRS-1 (S323) polyclonal antibody detects endogenous levels of IRS-1 only when phosphorylated at Ser323.

BiowMW :~ 180 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral elF4 and p70 S6 Kinase,Insulin &Glucose Signaling,

Alternative Name :Insulin receptor substrate 1; IRS-1; IRS1

Immunogen :Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 323.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of p-IRS-1 (S323) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:L02 treated with PBS(1×PBS,PH7.4) for 1 houre whole cell lysate Lane3:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 5min whole cell lysate Lane4:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 10min whole cell lysate Lane5:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 15min whole cell lysate

Immunohistochemistry (IHC) analyzes of p-IRS-1 (S323) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.