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Shc (phospho-Y349) polyclonal antibody（KU）

货号: BS4250

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4250

Product Name :Shc (phospho-Y349) polyclonal antibody（KU）

Swiss-Prot :P29353

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Critical components of this process include adapter proteins such as Shc and IRS-1 that lack detectable catalytic activity. These are immediate substrates of receptor tyrosine kinase activity and serve to physically link activated receptors to downstream signaling components. Whereas Shc has been implicated in signaling by diverse receptor families, IRS-1 serves primarily as the major insulin receptor substrate. Shc also participates in insulin signaling by linking the insulin receptor to Ras by forming complexes with the adapter protein GRB2 and Sos independently of IRS-1. A protein immunologically related to IRS-1, originally designated 4PS and now known as IRS-2, was shown to become highly tyrosine phosphorylated in response to IL-4 or IGF-1 in cells lacking IRS-1. An additional member of this family of signaling intermediates, Shb, is a SH2-containing protein with characteristic proline-rich domains.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Shc (Y349) polyclonal antibody detects endogenous levels of Shc protein only when phosphorylated at Tyr349.

BiowMW :~ 46, 52, 66 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1; SHC1; SHC; SHCA

Immunogen :Synthetic phosphopeptide derived from human Shc around the phosphorylation site of Tyrosine 349.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Shc (Y349) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HEK293T treated with EGF(100ng/ml,15 minutes) whole cell lysate Lane3:HEK293T treated with EGF(100ng/ml,30 minutes) whole cell lysate Lane4:The Kidney tissue lysate of Mouse Lane5:The Kidney tissue lysate of Rat

Immunohistochemistry (IHC) analyzes of p-Shc (Y349) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.