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XRCC5 (phospho-T714) polyclonal antibody（KU）

货号: BS4240

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4240

Product Name :XRCC5 (phospho-T714) polyclonal antibody（KU）

Swiss-Prot :P13010

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :XRCC5 encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-XRCC5 (T714) polyclonal antibody detects endogenous levels of XRCC5 protein only when phosphorylated at Thr714

BiowMW :~ 82 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :X-ray repair cross-complementing protein 5; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku80; Ku86; Ku-80; Ku-86; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); XRCC5; G22P2

Immunogen :Synthetic phosphopeptide derived from human XRCC5 around the phosphorylation site of Threonine 714.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-XRCC5 (T714) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate(40ug) Lane3:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug) Lane4:The Brain tissue lysate of Mouse(40ug) Lane5:The Uterus tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of p-Ku-80 (T714) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.