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IKKγ (phospho-S31) polyclonal antibody

货号: BS4238

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4238

Product Name :IKKγ (phospho-S31) polyclonal antibody

Swiss-Prot :Q9Y6K9

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Activation of NFkB requires that IkB be phosphorylated on specific serine residues, which results in targeted degradation of IkB. IkB kinase α (IKKα), previously designated CHUK, interacts with IkB-α and specifically phosphorylates I˚Bα on Serine 32 and 36, the sites that trigger its degradation. IKKα appears to be critical for NFkB activation in response to proinflammatory cytokines. Phosphorylation of IkB by IKKα is stimulated by the NFkB inducing kinase (NIK), which itself is a central regulator for NFkB activation in response to TNF and IL-1. The functional IKK complex contains three subunits, IKKα, IKKβ and IKKγ (also designated NEMO), and each appear to make essential contributions to IkB phosphorylation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-IKKγ (S31) polyclonal antibody detects endogenous levels of IKKγ protein only when phosphorylated at Ser31.

BiowMW :~ 48 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,STING Signaling,Inhibition of Apoptosis,

Alternative Name :NF-kappa-B essential modulator; NEMO; FIP-3; IkB kinase-associated protein 1; IKKAP1; Inhibitor of nuclear factor kappa-B kinase subunit gamma; I-kappa-B kinase subunit gamma; IKK-gamma; IKKG; IkB kinase subunit gamma; NF-kappa-B essential modifier; IKBKG; FIP3; NEMO; IKK-γ

Immunogen :Synthetic phosphopeptide derived from human IKKγ around the phosphorylation site of Serine 31.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-IKKγ (S31) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with TNFa(20ng/ml,15mins) Lane2:Raw264.7 cell lysate treated with TNFa(20ng/ml,15mins) Lane3:PC12 cell lysate treated with TNFa(20ng/ml,15mins)