产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

C/EBP α (phospho-S21) polyclonal antibody（KU）

货号: BS4217

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4217

Product Name :C/EBP α (phospho-S21) polyclonal antibody（KU）

Swiss-Prot :P49715

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :C/EBP α regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP α is a basic region/leucine zipper transcription factor selectively expressed during the differentiation of liver, adipose tissue, blood cells and the endocrine pancreas. C/EBP α uses a bipartite structural motif to bind DNA and appears to function exclusively in terminally differentiated, growtharrested cells. In the liver, C/EBP α is a transactivator of several genes, which are regulated by growth hormone. Growth hormone enhances not only the levels of C/EBP α mRNA and protein, but also the DNA-binding activity of C/EBP α. C/EBP α functions as an important transcription factor that regulates different genes, including Prolactin gene expression.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-C/EBP α (S21) polyclonal antibody detects endogenous levels of C/EBP α protein only when phosphorylated at Ser21.

BiowMW :~ 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; C/EBP α; C/EBPα

Immunogen :Synthetic phosphopeptide derived from human C/EBP α around the phosphorylation site of Serine 21.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot (WB) analysis of C/EBP α (phospho-S21) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 5 minutes whole cell lysate Lane3:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 10 minutes whole cell lysate Lane4:L02 treated with PBS(1×PBS,PH7.4) for 1 houre then treated with DMEM(10%FBS) for 15 minutes whole cell lysate

Immunohistochemistry (IHC) analyzes of p-C/EBP α (S21) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.