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Vav1 (phospho-Y174) polyclonal antibody

货号: BS4204

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4204

Product Name :Vav1 (phospho-Y174) polyclonal antibody

Swiss-Prot :P15498

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. Contained within its amino terminus are a helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins; deletion of this region of p95Vav causes its oncogenic activation. In addition, p95Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of p95Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Vav1 (Y174) polyclonal antibody detects endogenous levels of Vav1 protein when phosphorylated at Tyr174.

BiowMW :~ 100 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,

Alternative Name :Proto-oncogene vav; VAV1; VAV

Immunogen :Synthetic phosphopeptide derived from human VaV1 around the phosphorylation site of Tyrosine 174.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Vav1 (Y174) pAb at 1:500 dilution Lane1:THP-1 whole cell lysate(40ug) Lane2:HuT78 whole cell lysate(40ug) Lane3:The Spleen tissue lysate of Mouse(40ug) Lane4:The Spleen tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of p-Vav (Y174) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Novel role of Vav1-Rac1 pathway in actin cytoskeleton regulation in interleukin-13-induced minimal change-like nephropathy

JournalClinical Science

ApplicationIF,WB

IF5.223

SpeciesRat,Human

PMID27707912