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Syk (phospho-Y525) polyclonal antibody（KU）

货号: BS4189

价格：￥ 1350

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS4189

Product Name :Syk (phospho-Y525) polyclonal antibody（KU）

Swiss-Prot :P43405

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Syk (spleen tyrosine kinase) is a 635 amino acid protein that contains one protein kinase domain and two SH2 domains. One of several members of the protein kinase superfamily, Syk functions as a positive effector of B cell antigen receptor (CD79)-stimulated responses, coupling CD79 with the movement of one calcium ion through one of two phospho-regulated pathways. Via its ability to influence CD79 activity and to control the movement of calicum through the cell, Syk plays an important role in a variety of cellular responses, including differentiation, phagocytosis, proliferation and B cell development. Syk expression is upregulated in T cell lymphoma, suggesting a possible role for Syk in tumorigenesis. Two isoforms of Syk, designated short and long, exist due to alternative splicing events.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Syk (Y525) polyclonal antibody detects endogenous levels of Syk protein only when phosphorylated at Tyr525.

BiowMW :~ 69 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,B cell receptor signaling,

Alternative Name :Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK

Immunogen :Synthetic phosphopeptide derived from human Syk around the phosphorylation site of Tyrosine 525.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Syk (Y525) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate(40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug) Lane4:HEK293T treated with UV for 5 minutes then repair for 16 hours whole cell lysate(40ug) Lane5:HEK293T treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug) Lane6:The Lung tissue lysate of Rat(40ug) Lane7:The Uterus tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of p-Syk (Y525) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.