产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Stat4 (phospho-Y693) polyclonal antibody（KU）

货号: BS4183

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4183

Product Name :Stat4 (phospho-Y693) polyclonal antibody（KU）

Swiss-Prot :Q14765

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Stat4 (Y693) polyclonal antibody detects endogenous levels of Stat4 protein only when phosphorylated at Tyr693

BiowMW :~ 81 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Jak Stat ：IL-6 Receptor Signaling,

Alternative Name :Signal transducer and activator of transcription 4; Stat-4; Stat 4

Immunogen :Synthetic phosphopeptide derived from human Stat4 around the phosphorylation site of Tyrosine 693.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Stat4 (Y693) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:MCF-7 treated with IFN-γ(100ng/ml,15 minutes) whole cell lysate(40ug) Lane3:MCF-7 treated with IFN-γ(100ng/ml,30 minutes) whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-STAT4 (Y693) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Arctigenin exerts anti-colitis efficacy through inhibiting the differentiation of Th1 and Th17 cells via an mTORC1-dependent pathway

JournalBiochemical Pharmacology

ApplicationWB

IF4.825

SpeciesHuman,Mouse

PMID26074264