产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Stat3 (phospho-Y705) polyclonal antibody

货号: BS4181

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4181

Product Name :Stat3 (phospho-Y705) polyclonal antibody

Swiss-Prot :P40763

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Stat3 (Y705) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Tyr705

BiowMW :~ 88 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,Jak Stat ：IL-6 Receptor Signaling,

Alternative Name :Signal transducer and activator of transcription 3; Acute-phase response factor; STAT-3; APRF

Immunogen :Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Tyrosine 705.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of Stat3 (phospho-Y705) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate(40μg) Lane2:Hela treated with IFN-γ(100ng/ml, 30min) whole cell lysate(40μg) Lane3:Jurkat whole cell lysate(40μg) Lane4:Jurkat treated with H2O2(100nM/ml, 30min) whole cell lysate(40μg)

Immunohistochemistry (IHC) analyzes of p-STAT3 (Y705) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Promoting effects of IL‑23 on myocardial ischemia and reperfusion are associated with increased expression of IL‑17A and upregulation of the JAK2‑STAT3 signaling pathway

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesRat

PMID29039526
Uterine glands coordinate on-time embryo implantation and impact endometrial decidualization for pregnancy success

JournalNature Communications

ApplicationIHC

IF12.121

SpeciesMouse

PMID29934619
IL-9-triggered lncRNA Gm13568 regulates Notch1 in astrocytes through interaction with CBP/P300: contribute to the pathogenesis of experimental autoimmune encephalomyelitis

JournalJournal of Neuroinflammation

ApplicationWB

IF8.322

SpeciesMouse

PMID33971906
MiR‐409‐3p and MiR‐1896 co‐operatively participate in IL‐17‐induced inflammatory cytokine production in astrocytes and pathogenesis of EAE mice via targeting SOCS3/STAT3 signaling

JournalGlia

ApplicationWB

IF5.829

SpeciesMouse

PMID
PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization

JournalFrontiers in Pharmacology

ApplicationWB

IF5.81

SpeciesMouse

PMID33967779