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SRF (phospho-S103) polyclonal antibody（KU）

货号: BS4177

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4177

Product Name :SRF (phospho-S103) polyclonal antibody（KU）

Swiss-Prot :P11831

Host :Rabbit

Reactivity :Human

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1) have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-SRF (S103) polyclonal antibody detects endogenous levels of SRF protein when phosphorylated at Ser103.

BiowMW :~ 52 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Serum response factor

Immunogen :Synthetic phosphopeptide derived from human SRF around the phosphorylation site of Serine 103.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of SRF (phospho-S103) polyclonal antibody at 1:500 dilution Lane1:H1792 whole cell lysate(40ug) Lane2:SK-OVCAR3 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-SRF (S103) pAb in paraffin-embedded human lung cancer tissue.

Serum response factor provokes epithelial-mesenchymal transition in renal tubular epithelial cells of diabetic nephropathy

JournalPhysiological Genomics

ApplicationWB

IF3.107

SpeciesRat,Human

PMID27260841
Serum response factor induces endothelial-mesenchymal transition in glomerular endothelial cells to aggravate proteinuria in diabetic nephropathy

JournalPhysiological Genomics

ApplicationWB

IF3.107

SpeciesRat

PMID27565710
Crucial role of serum response factor in renal tubular epithelial cell epithelial-mesenchymal transition in hyperuricemic nephropathy

JournalAging-US

ApplicationWB

IF4.831

SpeciesRat

PMID31774735
Serum response factor increases renal cell carcinoma migration and invasion through promoting epithelial–mesenchymal transition

JournalInternational Journal Of Urology

ApplicationWB

IF2.445

SpeciesMouse,Human

PMID32524697