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Smad3 (phospho-S425) polyclonal antibody(KU)

货号: BS4173
价格 1350
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS4173
Product Name :Smad3 (phospho-S425) polyclonal antibody(KU)
Swiss-Prot :P84022
Host :Rabbit
Reactivity :Human,Mouse,Rat
Applications :WB IHC IF
Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
Background :Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of GFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-Smad3 (S425) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Ser425.
BiowMW :~ 56 kDa
Note :For research use only, not for use in diagnostic procedure.
Pathway :Tumor Angiogenesis,Cell Cycle Control G1 S Checkpoint,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling ,
Alternative Name :Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3
Immunogen :Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 425.
Conjugate :Unconjugated
Modification :Phosphorylated
Immunohistochemistry (IHC) analyzes of p-Smad3 (S425) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Immunofluorescence analysis of A549 cells using Smad3 (phospho-S425) pAb at dilution of 1:50 (40x lens).
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    JournalOncotarget
    ApplicationWB
    IF0
    SpeciesHuman
  • Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice
    JournalJournal Of Agricultural And Food Chemistry
    ApplicationWB
    IF3.571
    SpeciesMouse

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com