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SHP-1 (phospho-Y536) polyclonal antibody

货号: BS4169

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4169

Product Name :SHP-1 (phospho-Y536) polyclonal antibody

Swiss-Prot :P29350

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R β chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-SHP-1 (Y536) polyclonal antibody detects endogenous levels of SHP-1 protein only when phosphorylated at Tyr536.

BiowMW :~ 68 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :B cell receptor signaling,

Alternative Name :Tyrosine-protein phosphatase non-receptor type 6; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; PTP-1C; Protein-tyrosine phosphatase SHP-1; SH-PTP1; PTPN6; HCP; PTP1C

Immunogen :Synthetic phosphopeptide derived from human SHP-1 around the phosphorylation site of Tyrosine 536.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of SHP-1 (phospho-Y536) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:H1792 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:3T3-L1 whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of SHP-1 (phospho-Y536) polyclonal antibody in paraffin-embedded human breast carcinoma tissue at 1:100.

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ApplicationWB

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