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MEK-4/ MAP2K4 (phospho-S80) polyclonal antibody（KU）

货号: BS4167

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS4167

Product Name :MEK-4/ MAP2K4 (phospho-S80) polyclonal antibody（KU）

Swiss-Prot :P45985

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-MEK-4/ MAP2K4 (S80) polyclonal antibody detects endogenous levels of MEK-4 protein only when phosphorylated at Ser80.

BiowMW :~ 44 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :SAPK JNK Signaling Cascades,Signaling Pathways Activating p38 MAP KinaseMAPK Erk in Growth and Differentiation Pathway,

Alternative Name :Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK; MAP2K4; JNKK1; MEK4; MKK4;; PRKMK4; SEK1; SERK1; SKK1

Immunogen :Synthetic phosphopeptide derived from human MEK-4 around the phosphorylation site of Serine 80.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of MEK-4 (phospho-S80) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate Lane2:L02 treated with EGF(0.1ng/ml, 5min) whole cell lysate Lane3:L02 treated with EGF(0.1ng/ml, 10min) whole cell lysate Lane4:L02 treated with EGF(0.1ng/ml, 15min) whole cell lysate

Immunohistochemistry (IHC) analyzes of p-MEK-4/ MAP2K4 (S80) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.