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Rb (phospho-S795) polyclonal antibody（KU）

货号: BS4165

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS4165

Product Name :Rb (phospho-S795) polyclonal antibody（KU）

Swiss-Prot :P06400

Host :Rabbit

Reactivity :Human

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p-Rb (S795) polyclonal antibody detects endogenous levels of Rb protein only when phosphorylated at Ser795

BiowMW :~ 106 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,

Alternative Name :Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1

Immunogen :Synthetic phosphopeptide derived from human Rb around the phosphorylation site of Serine 795.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of p-Rb (S795) pAb at 1:500 dilution Lane1:SGC7901 whole cell lysate(40ug) Lane2:SGC7901 treated with PBS (1×PBS,PH7.4) for 3 hours whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p-Rb (S795) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Teng-Long-Bu-Zhong-Tang, a Chinese herbal formula, enhances anticancer effects of 5 - Fluorouracil in CT26 colon carcinoma

JournalBMC Complementary and Alternative Medicine

ApplicationIHC,WB

IF2.479

SpeciesMouse

PMID23758730
Tris (2-chloroethyl) phosphate induces senescence-like phenotype of hepatocytes via the p21Waf1/Cip1-Rb pathway in a p53-independent manner

JournalEnvironmental Toxicology And Pharmacology

ApplicationWB

IF4.86

SpeciesHuman

PMID28886428