Product Name :Rb (phospho-S780) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :IHC: 1:50~1:200
IF: 1:50~1:200
Background :The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, 2, 3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-Rb (S780) polyclonal antibody detects endogenous levels of Rb protein only when phosphorylated at Ser780.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Cell Cycle Control G1 S Checkpoint,
Alternative Name :Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110; RB1
Immunogen :Synthetic phosphopeptide derived from human Rb around the phosphorylation site of Serine 780.
Modification :Phosphorylation
