Product Name :Raf-1 (phospho-S259) polyclonal antibody
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:500~1:1000
IHC: 1:50~1:200
Background :Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :p-Raf1 (S259) polyclonal antibody detects endogenous levels of Raf1 protein only when phosphorylated at Ser259
Note :For research use only, not for use in diagnostic procedure.
Pathway :Inhibition of Apoptosis,G Protein-coupled Receptors Signaling to MAPK Erk,hippo Signaling,MAPK Erk in Growth and Differentiation Pathway,
Alternative Name :RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF
Immunogen :Synthetic phosphopeptide derived from human Raf-1 around the phosphorylation site of Serine 259.
Modification :Phosphorylation
